PDBe 3tuj

X-ray diffraction
4Å resolution

Inward facing conformations of the MetNI methionine ABC transporter: DM crystal form

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Methionine import ATP-binding protein MetN Chains: C, D
Molecule details ›
Chains: C, D
Length: 366 amino acids
Theoretical weight: 40.99 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P30750 (Residues: 1-343; Coverage: 100%)
Gene names: JW0195, abc, b0199, metN
Sequence domains:
Structure domains:
D-methionine transport system permease protein MetI Chains: A, B
Molecule details ›
Chains: A, B
Length: 217 amino acids
Theoretical weight: 23.74 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P31547 (Residues: 1-217; Coverage: 100%)
Gene names: JW0194, b0198, metI, yaeE
Sequence domains: Binding-protein-dependent transport system inner membrane component
Structure domains: MetI-like

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: I222
Unit cell:
a: 155.75Å b: 164.13Å c: 212.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.262 0.259 0.295
Expression system: Escherichia coli BL21