› 3tq6

X-ray diffraction
2.45Å resolution

Crystal structure of human mitochondrial transcription factor A, TFAM or mtTFA, bound to the light strand promoter LSP

Released:
DOI: 10.2210/pdb3tq6/pdb
PDB entry 3tq6 coloured by chain, front view.
PDB entry 3tq6 coloured by chain, side view.
PDB entry 3tq6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Human mitochondrial transcription factor A induces a U-turn structure in the light strand promoter.
Rubio-Cosials A, Sidow JF, Jiménez-Menéndez N, Fernández-Millán P, Montoya J, Jacobs HT, Coll M, Bernadó P, Solà M
Nat Struct Mol Biol 18 1281-9 (2011)
PMID: 22037172

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116942 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcription factor A, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 214 amino acids
Theoretical weight: 25.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q00059 (Residues: 43-246; Coverage: 83%)
Gene names: TCF6, TCF6L2, TFAM
Sequence domains:
Structure domains: High mobility group box domain
DNA (5'-D(*TP*AP*AP*CP*AP*GP*TP*CP*AP*CP*CP*CP*CP*CP*CP*AP*AP*CP*(BRU)P*AP*AP*C)-3') Chains: C, E
Molecule details ›
Chains: C, E
Length: 22 nucleotides
Theoretical weight: 6.66 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*GP*TP*TP*AP*GP*TP*TP*GP*GP*GP*GP*GP*GP*TP*GP*AP*CP*TP*GP*TP*TP*A)-3') Chains: D, F
Molecule details ›
Chains: D, F
Length: 22 nucleotides
Theoretical weight: 6.91 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand 1PE 1 x 1PE
Ligand PO4 1 x PO4
Ligand PEG 1 x PEG
1 modified residue:
Ligand BRU 2 x BRU

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21212
Unit cell:
a: 113.9Å b: 117.2Å c: 56.53Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.182 0.228
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

35 review citations

Mitochondrial form and function.
Friedman et al. (2014)
34 more

18 mentions without citation

Mitochondrial DNA: Epigenetics and environment.
Sharma et al. (2019)
17 more