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structure of the regulatory fragment of sacchromyces cerevisiae ampk in complex with NADH

X-ray diffraction
2.5Å resolution
PDB entry 3te5 coloured by chain, front view.
PDB entry 3te5 coloured by chain, side view.
PDB entry 3te5 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Assembly composition:
hetero trimer (preferred)
Entry contents: 3 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Snf1 protein kinase complex variant SIP2
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Multimeric state: hetero trimer
Accessible surface area: 25659.84 Å2
Buried surface area: 11269.14 Å2
Dissociation area: 2,772.7 Å2
Dissociation energy (ΔGdiss): 34.35 kcal/mol
Dissociation entropy (TΔSdiss): 13.71 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-139224
Complex Portal ID: CPX-2800

Macromolecules

Carbon catabolite-derepressing protein kinase
Chain: A
Length: 179 amino acids
Theoretical weight: 20.45 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P06782 (Residues: 457-633; Coverage: 28%)
Gene names: CAT1, CCR1, D8035.20, GLC2, PAS14, SNF1, YDR477W
Pfam: Adenylate sensor of SNF1-like protein kinase
InterPro:
CATH: Kinase associated domain 1, KA1
Carbon catabolite-derepressing protein kinase in PDB entry 3te5, assembly 1, front view.
Carbon catabolite-derepressing protein kinase in PDB entry 3te5, assembly 1, side view.
Carbon catabolite-derepressing protein kinase in PDB entry 3te5, assembly 1, top view.
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SNF1 protein kinase subunit beta-2
Chain: B
Length: 113 amino acids
Theoretical weight: 13.04 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P34164 (Residues: 304-415; Coverage: 27%)
Gene names: G1155, SIP2, SPM2, YGL208W
Pfam: 5'-AMP-activated protein kinase beta subunit, interaction domain
InterPro:
CATH: N-terminal domain of TfIIb
SNF1 protein kinase subunit beta-2 in PDB entry 3te5, assembly 1, front view.
SNF1 protein kinase subunit beta-2 in PDB entry 3te5, assembly 1, side view.
SNF1 protein kinase subunit beta-2 in PDB entry 3te5, assembly 1, top view.
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5'-AMP-activated protein kinase subunit gamma
Chain: C
Length: 323 amino acids
Theoretical weight: 36.51 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P12904 (Residues: 2-322; Coverage: 100%)
Gene names: CAT3, SCI1, SNF4, YGL115W
Pfam: CBS domain
InterPro:
CATH: CBS-domain
5'-AMP-activated protein kinase subunit gamma in PDB entry 3te5, assembly 1, front view.
5'-AMP-activated protein kinase subunit gamma in PDB entry 3te5, assembly 1, side view.
5'-AMP-activated protein kinase subunit gamma in PDB entry 3te5, assembly 1, top view.
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