› 3tbc

X-ray diffraction
2.7Å resolution

Small laccase from Streptomyces viridosporus T7A; alternate crystal form complexed with acetovanillone.

Released:
DOI: 10.2210/pdb3tbc/pdb
PDB entry 3tbc coloured by chain, front view.
PDB entry 3tbc coloured by chain, side view.
PDB entry 3tbc coloured by chain, top view.
Source organism: Streptomyces viridosporus
Primary publication:
Roles of small laccases from Streptomyces in lignin degradation.
Majumdar S, Lukk T, Solbiati JO, Bauer S, Nair SK, Cronan JE, Gerlt JA
Biochemistry 53 4047-58 (2014)
PMID: 24870309

Function and Biology Details

Reaction catalysed: 
4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo trimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-125391 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Small laccase, two-domain laccase, multi-copper oxidase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 313 amino acids
Theoretical weight: 34.21 KDa
Source organism: Streptomyces viridosporus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: J9PBR2 (Residues: 1-313; Coverage: 100%)
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

5 bound ligands:
Ligand CU 12 x CU
Ligand OXY 2 x OXY
Ligand PO4 7 x PO4
Ligand I75 1 x I75
Ligand TAM 1 x TAM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P3121
Unit cell:
a: 127.02Å b: 127.02Å c: 155.54Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.16 0.157 0.21
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

19 review citations

Lignocellulose degradation mechanisms across the Tree of Life.
Cragg et al. (2015)
18 more

1 mention without citation

Secretory expression of recombinant small laccase genes in Gram-positive bacteria.
Välimets et al. (2023)