› 3t7e

X-ray diffraction
2.25Å resolution

Atg8 transfer from Atg7 to Atg3: a distinctive E1-E2 architecture and mechanism in the autophagy pathway

Released:
DOI: 10.2210/pdb3t7e/pdb
PDB entry 3t7e coloured by chain, front view.
PDB entry 3t7e coloured by chain, side view.
PDB entry 3t7e coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Atg8 transfer from Atg7 to Atg3: a distinctive E1-E2 architecture and mechanism in the autophagy pathway.
Taherbhoy AM, Tait SW, Kaiser SE, Williams AH, Deng A, Nourse A, Hammel M, Kurinov I, Rock CO, Green DR, Schulman BA
Mol Cell 44 451-61 (2011)
PMID: 22055190

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153753 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin-like modifier-activating enzyme ATG7 Chain: A
Molecule details ›
Chain: A
Length: 344 amino acids
Theoretical weight: 38.48 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P38862 (Residues: 289-630; Coverage: 54%)
Gene names: APG7, ATG7, CVT2, YHR171W
Sequence domains: ThiF family
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P43212
Unit cell:
a: 66.556Å b: 66.556Å c: 222.268Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.193 0.233
Expression system: Escherichia coli

Quick links

Citations

31 review citations

The machinery of macroautophagy.
Feng et al. (2014)
30 more

5 mentions without citation

Accurate SAXS profile computation and its assessment by contrast variation experiments.
Schneidman-Duhovny et al. (2013)
4 more