PDBe 3t7e

X-ray diffraction
2.25Å resolution

Atg8 transfer from Atg7 to Atg3: a distinctive E1-E2 architecture and mechanism in the autophagy pathway

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin-like modifier-activating enzyme ATG7 Chain: A
Molecule details ›
Chain: A
Length: 344 amino acids
Theoretical weight: 38.48 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P38862 (Residues: 289-630; Coverage: 54%)
Gene names: APG7, ATG7, CVT2, YHR171W
Sequence domains: ThiF family
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P43212
Unit cell:
a: 66.556Å b: 66.556Å c: 222.268Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.193 0.233
Expression system: Escherichia coli