PDBe 3t3q

X-ray diffraction
2.1Å resolution

Human Cytochrome P450 2A6 I208S/I300F/G301A/S369G in complex with Pilocarpine

Released:

Function and Biology Details

Reaction catalysed:
RH + [reduced NADPH--hemoprotein reductase] + O(2) = ROH + [oxidized NADPH--hemoprotein reductase] + H(2)O
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 2A6 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 476 amino acids
Theoretical weight: 54.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P11509 (Residues: 29-494; Coverage: 94%)
Gene names: CYP2A3, CYP2A6
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 4 x HEM
1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 70.952Å b: 159.836Å c: 103.855Å
α: 90° β: 91.79° γ: 90°
R-values:
R R work R free
0.214 0.211 0.257
Expression system: Escherichia coli