› 3t06

X-ray diffraction
2.84Å resolution

Crystal Structure of the DH/PH fragment of PDZRHOGEF with N-terminal regulatory elements in complex with Human RhoA

Released:
DOI: 10.2210/pdb3t06/pdb
PDB entry 3t06 coloured by chain, front view.
PDB entry 3t06 coloured by chain, side view.
PDB entry 3t06 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Insights into the molecular activation mechanism of the RhoA-specific guanine nucleotide exchange factor, PDZRhoGEF.
Bielnicki JA, Shkumatov AV, Derewenda U, Somlyo AV, Svergun DI, Derewenda ZS
J Biol Chem 286 35163-75 (2011)
PMID: 21816819

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127285 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Rho guanine nucleotide exchange factor 11 Chains: A, E
Molecule details ›
Chains: A, E
Length: 418 amino acids
Theoretical weight: 48.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O15085 (Residues: 672-1081; Coverage: 27%)
Gene names: ARHGEF11, KIAA0380
Sequence domains:
Structure domains:
Transforming protein RhoA Chains: B, F
Molecule details ›
Chains: B, F
Length: 178 amino acids
Theoretical weight: 20.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61586 (Residues: 3-180; Coverage: 92%)
Gene names: ARH12, ARHA, RHO12, RHOA
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 84.37Å b: 118.63Å c: 92.443Å
α: 90° β: 113.61° γ: 90°
R-values:
R R work R free
0.21 0.208 0.257
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The emerging role of guanine nucleotide exchange factors in ALS and other neurodegenerative diseases.
Droppelmann et al. (2014)
14 other articles

8 mentions without citation

Structural Insights into the Regulation Mechanism of Small GTPases by GEFs.
Toma-Fukai et al. (2019)
7 more