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Crystal structure of BBBB+UDP+Gal with MPD as the cryoprotectant

X-ray diffraction
1.55Å resolution
PDB entry 3sxd coloured by chain, front view.
PDB entry 3sxd coloured by chain, side view.
PDB entry 3sxd coloured by chain, top view.
Source organism: Homo sapiens
Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase soluble form
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Multimeric state: monomeric
Accessible surface area: 13571.28 Å2
Buried surface area: 1367.91 Å2
Dissociation area: 136.36 Å2
Dissociation energy (ΔGdiss): -4.04 kcal/mol
Dissociation entropy (TΔSdiss): 1.46 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-147725
Assembly 2
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Assembly Name: Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase soluble form
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Multimeric state: homo dimer
Accessible surface area: 21845.76 Å2
Buried surface area: 8025.02 Å2
Dissociation area: 2,644.94 Å2
Dissociation energy (ΔGdiss): 27.47 kcal/mol
Dissociation entropy (TΔSdiss): 13.8 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-147726

Macromolecules

Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase soluble form
Chain: A
Length: 293 amino acids
Theoretical weight: 34.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P16442 (Residues: 64-354; Coverage: 82%)
Gene name: ABO
Pfam: Glycosyltransferase family 6
InterPro:
CATH: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A
Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase soluble form in PDB entry 3sxd, assembly 1, front view.
Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase soluble form in PDB entry 3sxd, assembly 1, side view.
Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase soluble form in PDB entry 3sxd, assembly 1, top view.
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