PDBe 3ssb

X-ray diffraction
1.8Å resolution

Structure of Insect Metalloproteinase Inhibitor in Complex with Thermolysin

Released:

Function and Biology Details

Reaction catalysed:
Preferential cleavage: Xaa-|-Leu > Xaa-|-Phe. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Thermolysin Chains: A, B
Molecule details ›
Chains: A, B
Length: 316 amino acids
Theoretical weight: 34.36 KDa
Source organism: Bacillus thermoproteolyticus
UniProt:
  • Canonical: P00800 (Residues: 233-548; Coverage: 61%)
Gene name: npr
Sequence domains:
Structure domains:
IMPI alpha Chains: C, D
Molecule details ›
Chains: C, D
Length: 40 amino acids
Theoretical weight: 4.29 KDa
Source organism: Galleria mellonella
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P82176 (Residues: 19-56; Coverage: 25%)
Gene name: IMPI
IMPI alpha Chains: I, J
Molecule details ›
Chains: I, J
Length: 32 amino acids
Theoretical weight: 3.68 KDa
Source organism: Galleria mellonella
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P82176 (Residues: 57-88; Coverage: 21%)
Gene name: IMPI

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 45.64Å b: 78.37Å c: 92.29Å
α: 90° β: 90.08° γ: 90°
R-values:
R R work R free
0.159 0.159 0.193
Expression system: Escherichia coli BL21(DE3)