PDBe 3sn6

X-ray diffraction
3.2Å resolution

Function and Biology Details

Reaction catalysed:
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 380 amino acids
Theoretical weight: 44.37 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P04896 (Residues: 1-394; Coverage: 96%)
  • Best match: P04896-2 (Residues: 1-380)
Gene names: GNAS, GNAS1
Sequence domains: G-protein alpha subunit
Structure domains:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 351 amino acids
Theoretical weight: 38.74 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P54311 (Residues: 2-340; Coverage: 100%)
Gene name: Gnb1
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 68 amino acids
Theoretical weight: 7.56 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63212 (Residues: 1-68; Coverage: 96%)
Gene name: GNG2
Structure domains: Transducin (heterotrimeric G protein), gamma chain
Beta-2 adrenergic receptor; Endolysin Chain: R
Molecule details ›
Chain: R
Length: 514 amino acids
Theoretical weight: 58.3 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P07550 (Residues: 29-365; Coverage: 82%)
  • Canonical: P00720 (Residues: 2-161; Coverage: 98%)
Gene names: ADRB2, ADRB2R, B2AR, E
Sequence domains:
Structure domains:
Camelid antibody VHH fragment Chain: N
Molecule details ›
Chain: N
Length: 138 amino acids
Theoretical weight: 15.14 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B, null
Spacegroup: P21
Unit cell:
a: 119.339Å b: 64.555Å c: 131.24Å
α: 90° β: 91.67° γ: 90°
R-values:
R R work R free
0.228 0.226 0.277
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli