PDBe 3sn5

X-ray diffraction
2.75Å resolution

Crystal structure of human CYP7A1 in complex with cholest-4-en-3-one

Source organism: Homo sapiens
Entry authors: Strushkevich N, Tempel W, MacKenzie F, Wernimont AK, Arrowsmith CH, Edwards AM, Bountra C, Weigelt J, Park H, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed:
Cholesterol + [reduced NADPH--hemoprotein reductase] + O(2) = 7-alpha-hydroxycholesterol + [oxidized NADPH--hemoprotein reductase] + H(2)O
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Cholesterol 7-alpha-monooxygenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 491 amino acids
Theoretical weight: 56.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P22680 (Residues: 25-503; Coverage: 95%)
Gene names: CYP7, CYP7A1
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments

Cofactor: Ligand HEM 2 x HEM
2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 56.16Å b: 137.63Å c: 160.15Å
α: 90° β: 90° γ: 90°
R R work R free
0.184 0.182 0.22
Expression system: Escherichia coli