› 3slh

X-ray diffraction
1.7Å resolution

1.70 Angstrom resolution structure of 3-phosphoshikimate 1-carboxyvinyltransferase (AroA) from Coxiella burnetii in complex with shikimate-3-phosphate and glyphosate

Released:
DOI: 10.2210/pdb3slh/pdb
PDB entry 3slh coloured by chain, front view.
PDB entry 3slh coloured by chain, side view.
PDB entry 3slh coloured by chain, top view.
Source organism: Coxiella burnetii RSA 493
Entry authors: Light SH, Minasov G, Filippova EV, Krishna SN, Shuvalova L, Papazisi L, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182936 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-phosphoshikimate 1-carboxyvinyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 441 amino acids
Theoretical weight: 46.75 KDa
Source organism: Coxiella burnetii RSA 493
Expression system: Escherichia coli
UniProt:
  • Canonical: Q83E11 (Residues: 1-438; Coverage: 100%)
Gene names: CBU_0526, aroA
Sequence domains: EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Structure domains: Enolpyruvate transferase domain

Ligands and Environments

11 bound ligands:
Ligand BME 5 x BME
Ligand PO4 4 x PO4
Ligand S3P 4 x S3P
Ligand GPJ 5 x GPJ
Ligand SKM 4 x SKM
Ligand EDO 4 x EDO
Ligand PEG 14 x PEG
Ligand PGE 1 x PGE
Ligand 1PE 1 x 1PE
Ligand CL 2 x CL
Ligand PG4 3 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P21
Unit cell:
a: 46.252Å b: 186.638Å c: 95.794Å
α: 90° β: 91.51° γ: 90°
R-values:
R R work R free
0.151 0.149 0.182
Expression system: Escherichia coli

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