› 3sky

X-ray diffraction
2.1Å resolution

2.1A crystal structure of the phosphate bound ATP binding domain of Archaeoglobus fulgidus COPB

Released:
DOI: 10.2210/pdb3sky/pdb
PDB entry 3sky coloured by chain, front view.
PDB entry 3sky coloured by chain, side view.
PDB entry 3sky coloured by chain, top view.
Source organism: Archaeoglobus fulgidus DSM 4304
Primary publication:
Conformations of the apo-, substrate-bound and phosphate-bound ATP-binding domain of the Cu(II) ATPase CopB illustrate coupling of domain movement to the catalytic cycle.
Jayakanthan S, Roberts SA, Weichsel A, Argüello JM, McEvoy MM
Biosci Rep 32 443-53 (2012)
PMID: 22663904

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + Cu(2+)(Side 1) = ADP + phosphate + Cu(2+)(Side 2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128210 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Copper-exporting P-type ATPase B Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 30.14 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Escherichia coli
UniProt:
  • Canonical: O30085 (Residues: 371-636; Coverage: 39%)
Gene names: AF_0152, copB
Sequence domains: haloacid dehalogenase-like hydrolase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand PO4 1 x PO4
Ligand MPO 1 x MPO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Unit cell:
a: 35.761Å b: 62.909Å c: 106.539Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.214 0.264
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Diversity of the metal-transporting P1B-type ATPases.
Smith et al. (2014)
2 more

1 mention without citation

Conformations of the apo-, substrate-bound and phosphate-bound ATP-binding domain of the Cu(II) ATPase CopB illustrate coupling of domain movement to the catalytic cycle.
Jayakanthan et al. (2012)