› 3sgw

X-ray diffraction
1.7Å resolution

Crystal structure of ribose-5-phosphate isomerase B RpiB from Coccidioides immitis semi-covalently bound to malonic acid

Released:
DOI: 10.2210/pdb3sgw/pdb
PDB entry 3sgw coloured by chain, front view.
PDB entry 3sgw coloured by chain, side view.
PDB entry 3sgw coloured by chain, top view.
Source organism: Coccidioides immitis RS
Primary publication:
Structural characterization of a ribose-5-phosphate isomerase B from the pathogenic fungus Coccidioides immitis.
Edwards TE, Abramov AB, Smith ER, Baydo RO, Leonard JT, Leibly DJ, Thompkins KB, Clifton MC, Gardberg AS, Staker BL, Van Voorhis WC, Myler PJ, Stewart LJ
BMC Struct Biol 11 39 (2011)
PMID: 21995815

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-143525 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative ribose 5-phosphate isomerase Chain: A
Molecule details ›
Chain: A
Length: 184 amino acids
Theoretical weight: 19.71 KDa
Source organism: Coccidioides immitis RS
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CL19 (Residues: 1-163; Coverage: 100%)
Gene name: CIMG_07932
Sequence domains: Ribose/Galactose Isomerase
Structure domains: Sugar-phosphate isomerase, RpiB/LacA/LacB

Ligands and Environments

3 bound ligands:
Ligand MLA 1 x MLA
Ligand EDO 2 x EDO
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: F222
Unit cell:
a: 77.46Å b: 84.42Å c: 96.17Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.144 0.142 0.175
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Isomerases for biotransformation of D-hexoses.
Mu et al. (2015)
2 other articles

1 mention without citation

Structural characterization of a ribose-5-phosphate isomerase B from the pathogenic fungus Coccidioides immitis.
Edwards et al. (2011)