PDBe 3sf8

X-ray diffraction
1.56Å resolution

Structural insights into thiol stabilization of DJ-1

Released:
Source organism: Homo sapiens

Function and Biology Details

Reaction catalysed:
(1) An N(omega)-(1-hydroxy-2-oxopropyl)-[protein]-L-arginine + H(2)O = a [protein]-L-arginine + (R)-lactate. (2) An N(6)-(1-hydroxy-2-oxopropyl)-[protein]-L-lysine + H(2)O = a [protein]-L-lysine + (R)-lactate. (3) An S-(1-hydroxy-2-oxopropyl)-[protein]-L-cysteine + H(2)O = a [protein]-L-cysteine + (R)-lactate. 

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein/nucleic acid deglycase DJ-1 Chain: A
Molecule details ›
Chain: A
Length: 191 amino acids
Theoretical weight: 20.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99497 (Residues: 1-189; Coverage: 100%)
Gene name: PARK7
Sequence domains: DJ-1/PfpI family
Structure domains: Rossmann fold
Protein/nucleic acid deglycase DJ-1 Chain: B
Molecule details ›
Chain: B
Length: 191 amino acids
Theoretical weight: 20.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99497 (Residues: 1-189; Coverage: 100%)
Gene name: PARK7
Sequence domains: DJ-1/PfpI family
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: C2
Unit cell:
a: 141.88Å b: 41.831Å c: 63.344Å
α: 90° β: 115.79° γ: 90°
R-values:
R R work R free
0.174 0.172 0.211
Expression system: Escherichia coli