PDBe 3seq

X-ray diffraction
2.73Å resolution

Crystal structure of C176A mutant of glutamine-dependent NAD+ synthetase from M. tuberculosis in complex with AMPCPP and NaAD+

Released:

Function and Biology Details

Reaction catalysed:
ATP + deamido-NAD(+) + L-glutamine + H(2)O = AMP + diphosphate + NAD(+) + L-glutamate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamine-dependent NAD(+) synthetase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 680 amino acids
Theoretical weight: 74.82 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WJJ3 (Residues: 1-679; Coverage: 100%)
Gene names: MTCY428.08, Rv2438c, nadE
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P41212
Unit cell:
a: 178.166Å b: 178.166Å c: 214.907Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.163 0.212
Expression system: Escherichia coli BL21(DE3)