› 3saf

X-ray diffraction
2.5Å resolution

Crystal structure of the human RRP6 catalytic domain with D313N mutation in the active site

Released:
DOI: 10.2210/pdb3saf/pdb
PDB entry 3saf coloured by chain, front view.
PDB entry 3saf coloured by chain, side view.
PDB entry 3saf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Activities of human RRP6 and structure of the human RRP6 catalytic domain.
Januszyk K, Liu Q, Lima CD
RNA 17 1566-77 (2011)
PMID: 21705430

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169626 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Exosome complex component 10 Chains: A, B
Molecule details ›
Chains: A, B
Length: 428 amino acids
Theoretical weight: 50.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q01780 (Residues: 180-606; Coverage: 48%)
Gene names: EXOSC10, PMSCL, PMSCL2, RRP6
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand YT3 2 x YT3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P4
Unit cell:
a: 138.9Å b: 138.9Å c: 58.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.205 0.24
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

RNA decay machines: the exosome.
Chlebowski et al. (2013)
11 more

8 mentions without citation

The eukaryotic RNA exosome.
Januszyk et al. (2014)
7 more