Function and Biology

Crystal Structures of Glutaryl 7-Aminocephalosporanic Acid Acylase: Insight into Autoproteolytic Activation

Source organism: Pseudomonas sp. SY-77-1

EC 3.5.1.93: Glutaryl-7-aminocephalosporanic-acid acylase

Reaction catalysed:
(7R)-7-(4-carboxybutanamido)cephalosporanate + H(2)O = (7R)-7- aminocephalosporanate + glutarate.
Comments:
  • Forms 7-aminocephalosporanic acid, a key intermediate in the synthesis of cephem antibiotics.
  • It reacts only weakly with cephalosporin C.
Systematic name:
(7R)-7-(4-carboxybutanamido)cephalosporanate amidohydrolase
Alternative Name(s):
  • 7-beta-(4-carboxybutanamido)cephalosporanic acid acylase
  • Cephalosporin C acylase
  • Glutaryl-7-ACA acylase
  • CA
  • GCA
  • GA
  • Cephalosporin acylase
  • Glutaryl-7-aminocephalosporanic acid acylase
  • GL-7-ACA acylase

Sequence family

Pfam Protein family (Pfam)
PF01804
Domain description: Penicillin amidase
Occurring in:
  1. Glutaryl-7-aminocephalosporanic-acid acylase
1 copy of Pfam domain PF01804 (Penicillin amidase) in Glutaryl-7-aminocephalosporanic-acid acylase in PDB 3s8r.

InterPro InterPro annotations
IPR002692
Domain description: Penicillin/GL-7-ACA/AHL/aculeacin-A acylase
Occurring in:
  1. Glutaryl-7-aminocephalosporanic-acid acylase
IPR029055
Domain description: Nucleophile aminohydrolases, N-terminal
Occurring in:
  1. Glutaryl-7-aminocephalosporanic-acid acylase
IPR014395
Domain description: Penicillin/GL-7-ACA/AHL acylase
Occurring in:
  1. Glutaryl-7-aminocephalosporanic-acid acylase
IPR023343
Domain description: Penicillin amidase type, domain 1
Occurring in:
  1. Glutaryl-7-aminocephalosporanic-acid acylase

Structure domains

CATH CATH domains
3.60.20.10
Class: Alpha Beta
Architecture: 4-Layer Sandwich
Topology: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homology: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Occurring in:
  1. Glutaryl-7-aminocephalosporanic-acid acylase
1 copy of CATH domain 3.60.20.10 (Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1) in Glutaryl-7-aminocephalosporanic-acid acylase in PDB 3s8r.
2.30.120.10
Class: Mainly Beta
Architecture: Roll
Topology: Penicillin G acylase, beta-roll domain
Homology: Penicillin G acylase, beta-roll domain
Occurring in:
  1. Glutaryl-7-aminocephalosporanic-acid acylase
1 copy of CATH domain 2.30.120.10 (Penicillin G acylase, beta-roll domain) in Glutaryl-7-aminocephalosporanic-acid acylase in PDB 3s8r.
1.10.1400.10
Class: Mainly Alpha
Architecture: Orthogonal Bundle
Topology: Penicillin amidase (Acylase) alpha subunit, N-terminal domain
Homology: Penicillin amidase (Acylase) alpha subunit, N-terminal domain
Occurring in:
  1. Glutaryl-7-aminocephalosporanic-acid acylase
1 copy of CATH domain 1.10.1400.10 (Penicillin amidase (Acylase) alpha subunit, N-terminal domain) in Glutaryl-7-aminocephalosporanic-acid acylase in PDB 3s8r.
1.10.439.10
Class: Mainly Alpha
Architecture: Orthogonal Bundle
Topology: Penicillin Amidohydrolase; domain 1
Homology: Penicillin Amidohydrolase, domain 1
Occurring in:
  1. Glutaryl-7-aminocephalosporanic-acid acylase
1 copy of CATH domain 1.10.439.10 (Penicillin Amidohydrolase; domain 1) in Glutaryl-7-aminocephalosporanic-acid acylase in PDB 3s8r.