PDBe 3s8r

X-ray diffraction
2.5Å resolution

Crystal Structures of Glutaryl 7-Aminocephalosporanic Acid Acylase: Insight into Autoproteolytic Activation

Released:

Function and Biology Details

Reaction catalysed:
(7R)-7-(4-carboxybutanamido)cephalosporanate + H(2)O = (7R)-7- aminocephalosporanate + glutarate. 

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutaryl-7-aminocephalosporanic-acid acylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 697 amino acids
Theoretical weight: 77.58 KDa
Source organism: Pseudomonas sp. SY-77-1
Expression system: Escherichia coli
UniProt:
  • Canonical: P07662 (Residues: 30-720; Coverage: 100%)
Sequence domains: Penicillin amidase
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 6B
Spacegroup: C2
Unit cell:
a: 225.386Å b: 68.806Å c: 112.756Å
α: 90° β: 97.51° γ: 90°
R-values:
R R work R free
0.156 0.154 0.2
Expression system: Escherichia coli