› 3s8c

X-ray diffraction
2.77Å resolution

Structure of Yeast Ribonucleotide Reductase 1 R293A with AMPPNP and CDP

Released:
DOI: 10.2210/pdb3s8c/pdb
PDB entry 3s8c coloured by chain, front view.
PDB entry 3s8c coloured by chain, side view.
PDB entry 3s8c coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Entry authors: Ahmad MF, Kaushal PS, Wan Q, Wijeratna SR, Huang M, Dealwis CD

Function and Biology Details

Reaction catalysed: 
2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H(2)O = ribonucleoside diphosphate + thioredoxin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149283 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonucleoside-diphosphate reductase large chain 1 Chain: A
Molecule details ›
Chain: A
Length: 888 amino acids
Theoretical weight: 99.59 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P21524 (Residues: 1-888; Coverage: 100%)
Gene names: CRT7, RIR1, RNR1, SDS12, YER070W
Sequence domains:
Structure domains: Anaerobic Ribonucleotide-triphosphate Reductase Large Chain

Ligands and Environments

3 bound ligands:
Ligand ANP 1 x ANP
Ligand MG 1 x MG
Ligand CDP 1 x CDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21212
Unit cell:
a: 107.781Å b: 116.81Å c: 64.126Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.196 0.256
Expression system: Escherichia coli

Quick links