Function and Biology Details
Reactions catalysed:
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- Immunoglobulin C1-set
- MHC class I alpha chain, alpha1 alpha2 domains
- Beta-2-Microglobulin
- Immunoglobulin/major histocompatibility complex, conserved site
- Immunoglobulin-like domain
- Immunoglobulin-like domain superfamily
- Immunoglobulin-like fold
- MHC classes I/II-like antigen recognition protein
2 more domains
Structure analysis Details
Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-177757 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ig-like domain-containing protein
Molecule details ›
Chains: A, D
Length: 276 amino acids
Theoretical weight: 32.03 KDa
Source organism: Macaca mulatta
Expression system: Escherichia coli
UniProt:
Sequence domains:
Structure domains:
Length: 276 amino acids
Theoretical weight: 32.03 KDa
Source organism: Macaca mulatta
Expression system: Escherichia coli
UniProt:
- Canonical:
Q9GJ77 (Residues: 24-299; Coverage: 80%)
Sequence domains:
Structure domains:
Beta-2-microglobulin
Molecule details ›
Chains: B, E
Length: 99 amino acids
Theoretical weight: 11.66 KDa
Source organism: Macaca mulatta
Expression system: Escherichia coli
UniProt:
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Length: 99 amino acids
Theoretical weight: 11.66 KDa
Source organism: Macaca mulatta
Expression system: Escherichia coli
UniProt:
- Canonical: Q6V7J5 (Residues: 21-119; Coverage: 100%)
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Pol protein
Molecule details ›
Chains: C, F
Length: 8 amino acids
Theoretical weight: 1.03 KDa
Source organism: Simian immunodeficiency virus
Expression system: Not provided
UniProt:
Length: 8 amino acids
Theoretical weight: 1.03 KDa
Source organism: Simian immunodeficiency virus
Expression system: Not provided
UniProt:
- Canonical: Q5QGH9 (Residues: 372-379; Coverage: 1%)
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
RIGAKU
Spacegroup:
P1
Expression systems:
- Escherichia coli
- Not provided
