PDBe 3ruk

X-ray diffraction
2.6Å resolution

Human Cytochrome P450 CYP17A1 in complex with Abiraterone

Released:
Source organism: Homo sapiens

Function and Biology Details

Reactions catalysed:
A C(21)-steroid + [reduced NADPH--hemoprotein reductase] + O(2) = a 17-alpha-hydroxy-C(21)-steroid + [oxidized NADPH--hemoprotein reductase] + H(2)O. 
(1) 17-alpha-hydroxyprogesterone + [reduced NADPH--hemoprotein reductase] + O(2) = androstenedione + acetate + [oxidized NADPH--hemoprotein reductase] + H(2)O. (2) 17-alpha-hydroxypregnenolone + [reduced NADPH--hemoprotein reductase] + O(2) = 3-beta-hydroxyandrost-5-en-17-one + acetate + [oxidized NADPH- -hemoprotein reductase] + H(2)O. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Steroid 17-alpha-hydroxylase/17,20 lyase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 494 amino acids
Theoretical weight: 55.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05093 (Residues: 24-508; Coverage: 96%)
Gene names: CYP17, CYP17A1, S17AH
Sequence domains: Cytochrome P450
Structure domains: Cytochrome p450

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 85.813Å b: 152.098Å c: 172.882Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.218 0.291
Expression system: Escherichia coli