› 3rsp

X-ray diffraction
1.7Å resolution

STRUCTURE OF THE P93G VARIANT OF RIBONUCLEASE A

Released:
DOI: 10.2210/pdb3rsp/pdb
PDB entry 3rsp coloured by chain, front view.
PDB entry 3rsp coloured by chain, side view.
PDB entry 3rsp coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structure and stability of the P93G variant of ribonuclease A.
Schultz LW, Hargraves SR, Klink TA, Raines RT
Protein Sci 7 1620-5 (1998)
PMID: 9684895

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158390 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease pancreatic Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 13.67 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61823 (Residues: 27-150; Coverage: 100%)
Gene names: RNASE1, RNS1
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P3221
Unit cell:
a: 65.79Å b: 65.79Å c: 66.12Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.17 0.17 not available
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Back to the future: ribonuclease A.
Marshall et al. (2008)
11 other articles

3 mentions without citation

Hypervalent nonbonded interactions of a divalent sulfur atom. Implications in protein architecture and the functions.
Iwaoka et al. (2012)
2 more