› 3ror

X-ray diffraction
2Å resolution

Crystal structure of C105S mutant of Mycobacterium tuberculosis methionine aminopeptidase

Released:
DOI: 10.2210/pdb3ror/pdb
PDB entry 3ror coloured by chain, front view.
PDB entry 3ror coloured by chain, side view.
PDB entry 3ror coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Entry authors: Reddi R, Kishor C, Addlagatta A

Function and Biology Details

Reaction catalysed: 
Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161719 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methionine aminopeptidase 2 Chain: A
Molecule details ›
Chain: A
Length: 291 amino acids
Theoretical weight: 31.73 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WK19 (Residues: 1-285; Coverage: 100%)
Gene names: MTV003.07c, Rv2861c, map, mapB
Sequence domains: Metallopeptidase family M24
Structure domains: Creatinase/methionine aminopeptidase superfamily

Ligands and Environments

3 bound ligands:
Ligand CO 2 x CO
Ligand K 1 x K
Ligand BME 1 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21
Unit cell:
a: 49.22Å b: 48.66Å c: 55.72Å
α: 90° β: 95.16° γ: 90°
R-values:
R R work R free
0.214 0.211 0.274
Expression system: Escherichia coli

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