› 3ri6

X-ray diffraction
2.2Å resolution

A Novel Mechanism of Sulfur Transfer Catalyzed by O-Acetylhomoserine Sulfhydrylase in Methionine Biosynthetic Pathway of Wolinella succinogenes

Released:
DOI: 10.2210/pdb3ri6/pdb
PDB entry 3ri6 coloured by chain, front view.
PDB entry 3ri6 coloured by chain, side view.
PDB entry 3ri6 coloured by chain, top view.
Source organism: Wolinella succinogenes
Primary publication:
A novel mechanism of sulfur transfer catalyzed by O-acetylhomoserine sulfhydrylase in the methionine-biosynthetic pathway of Wolinella succinogenes.
Tran TH, Krishnamoorthy K, Begley TP, Ealick SE
Acta Crystallogr D Biol Crystallogr 67 831-8 (2011)
PMID: 21931214

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181710 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
O-ACETYLHOMOSERINE SULFHYDRYLASE Chains: A, B
Molecule details ›
Chains: A, B
Length: 430 amino acids
Theoretical weight: 47.61 KDa
Source organism: Wolinella succinogenes
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7M9C8 (Residues: 1-407; Coverage: 100%)
Gene name: WS1012
Sequence domains: Cys/Met metabolism PLP-dependent enzyme
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: C2
Unit cell:
a: 161.8Å b: 62.5Å c: 91.6Å
α: 90° β: 120.5° γ: 90°
R-values:
R R work R free
0.226 0.225 0.251
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Bacterial methionine biosynthesis.
Ferla et al. (2014)
5 other articles

2 mentions without citation

A novel mechanism of sulfur transfer catalyzed by O-acetylhomoserine sulfhydrylase in the methionine-biosynthetic pathway of Wolinella succinogenes.
Tran et al. (2011)
1 more