PDBe 3rdz

X-ray diffraction
2.26Å resolution

Crystal Structure of rBTI-trypsin complex at 2.26 angstrom resolution

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cationic trypsin Chains: A, B
Molecule details ›
Chains: A, B
Length: 223 amino acids
Theoretical weight: 23.32 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00760 (Residues: 24-246; Coverage: 97%)
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Proteinase inhibitor Chains: C, D
Molecule details ›
Chains: C, D
Length: 79 amino acids
Theoretical weight: 9.02 KDa
Source organism: Fagopyrum esculentum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9S9F3 (Residues: 1-69; Coverage: 100%)
Sequence domains: Potato inhibitor I family
Structure domains: Trypsin Inhibitor V, subunit A

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 66.709Å b: 50.236Å c: 84.492Å
α: 90° β: 95.06° γ: 90°
R-values:
R R work R free
0.184 0.182 0.226
Expression system: Escherichia coli