PDBe 3rc2

X-ray diffraction
1.8Å resolution

Crystal Structure of KijD10, a 3-ketoreductase from Actinomadura kijaniata in complex with TDP-benzene and NADP; open conformation

Released:

Function and Biology Details

Reaction catalysed:
dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose + NADP(+) = dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose + NADPH 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose 3-reductase Chain: A
Molecule details ›
Chain: A
Length: 350 amino acids
Theoretical weight: 39.26 KDa
Source organism: Actinomadura kijaniata
Expression system: Escherichia coli
UniProt:
  • Canonical: B3TMR8 (Residues: 1-332; Coverage: 100%)
Gene name: KijD10
Sequence domains: Oxidoreductase family, NAD-binding Rossmann fold
Structure domains:

Ligands and Environments


Cofactor: Ligand NAP 1 x NAP

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: I222
Unit cell:
a: 73.087Å b: 104.822Å c: 144.469Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.207 0.243
Expression system: Escherichia coli