› 3r8b

X-ray diffraction
2.95Å resolution

Crystal structure of Staphylococcal Enterotoxin B in complex with an affinity matured mouse TCR VBeta8.2 protein, G5-8

Released:
DOI: 10.2210/pdb3r8b/pdb
PDB entry 3r8b coloured by chain, front view.
PDB entry 3r8b coloured by chain, side view.
PDB entry 3r8b coloured by chain, top view.
Source organisms:
Primary publication:
Molecular basis of a million-fold affinity maturation process in a protein-protein interaction.
Bonsor DA, Postel S, Pierce BG, Wang N, Zhu P, Buonpane RA, Weng Z, Kranz DM, Sundberg EJ
J Mol Biol 411 321-8 (2011)
PMID: 21689661

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208431 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Enterotoxin type B Chains: A, C, E, G, I, K, M, O
Molecule details ›
Chains: A, C, E, G, I, K, M, O
Length: 239 amino acids
Theoretical weight: 28.41 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01552 (Residues: 28-266; Coverage: 100%)
Gene name: entB
Sequence domains:
Structure domains:
G5-8 Chains: B, D, F, H, J, L, N, P
Molecule details ›
Chains: B, D, F, H, J, L, N, P
Length: 125 amino acids
Theoretical weight: 13.82 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

3 bound ligands:
Ligand CL 14 x CL
Ligand SO4 1 x SO4
Ligand ZN 24 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P212121
Unit cell:
a: 109.954Å b: 160.37Å c: 186.265Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.246 0.265
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Computational design of protein-protein interactions.
Schreiber et al. (2013)
3 other articles

3 mentions without citation

Soluble T cell receptor Vβ domains engineered for high-affinity binding to staphylococcal or streptococcal superantigens.
Sharma et al. (2014)
2 more