› 3r77

X-ray diffraction
1.9Å resolution

Crystal structure of the D38A mutant of isochorismatase PhzD from Pseudomonas fluorescens 2-79 in complex with 2-amino-2-desoxyisochorismate ADIC

Released:
DOI: 10.2210/pdb3r77/pdb
PDB entry 3r77 coloured by chain, front view.
PDB entry 3r77 coloured by chain, side view.
PDB entry 3r77 coloured by chain, top view.
Source organism: Pseudomonas fluorescens
Primary publication:
Ligand binding induces an ammonia channel in 2-amino-2-desoxyisochorismate (ADIC) synthase PhzE.
Li QA, Mavrodi DV, Thomashow LS, Roessle M, Blankenfeldt W
J Biol Chem 286 18213-21 (2011)
PMID: 21454481

Function and Biology Details

Reaction catalysed: 
(2S)-2-amino-4-deoxychorismate + H(2)O = (5S,6S)-6-amino-5-hydroxycyclohexa-1,3-diene-1-carboxylate + pyruvate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175973 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phenazine biosynthesis protein PhzD Chains: A, B
Molecule details ›
Chains: A, B
Length: 209 amino acids
Theoretical weight: 23.25 KDa
Source organism: Pseudomonas fluorescens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q51790 (Residues: 1-207; Coverage: 100%)
Gene name: phzD
Sequence domains: Isochorismatase family
Structure domains: Isochorismatase-like

Ligands and Environments

2 bound ligands:
Ligand QLI 2 x QLI
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P212121
Unit cell:
a: 68.48Å b: 72.1Å c: 78.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.145 0.143 0.192
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Gates of enzymes.
Gora et al. (2013)
7 more

2 mentions without citation

The structural biology of phenazine biosynthesis.
Blankenfeldt et al. (2014)
1 more