PDBe 3qrq

X-ray diffraction
3.19Å resolution

Structure of Thermus Thermophilus Cse3 bound to an RNA representing a pre-cleavage complex

Released:

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA/RNA hybrid molecule
Macromolecules (2 distinct):
CRISPR-associated endoribonuclease Cse3 Chain: A
Molecule details ›
Chain: A
Length: 267 amino acids
Theoretical weight: 30 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53WG9 (Residues: 1-211; Coverage: 100%)
Gene names: TTHB192, cas6, cse3
Sequence domains: CRISPR associated protein
Structure domains:
RNA (5'-R(*GP*UP*CP*CP*CP*CP*AP*CP*GP*CP*GP*UP*GP*UP*GP*GP*GP*GP*A)-3') Chain: B
Molecule details ›
Chain: B
Length: 19 nucleotides
Theoretical weight: 6.11 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: I222
Unit cell:
a: 61.5Å b: 68.91Å c: 158.063Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.21 0.266
Expression systems:
  • Escherichia coli
  • Not provided