PDBe 3qrp

X-ray diffraction
2.35Å resolution

Structure of Thermus Thermophilus Cse3 bound to an RNA representing a product mimic complex

Released:

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
CRISPR-associated endoribonuclease Cse3 Chain: A
Molecule details ›
Chain: A
Length: 267 amino acids
Theoretical weight: 30 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53WG9 (Residues: 1-211; Coverage: 100%)
Gene names: TTHB192, cas6, cse3
Sequence domains: CRISPR associated protein
Structure domains:
RNA (5'-R(P*(U5P)P*GP*UP*GP*GP*GP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 7 nucleotides
Theoretical weight: 2.29 KDa
Source organism: Synthetic construct
Expression system: Not provided
RNA (5'-R(*GP*UP*CP*CP*CP*CP*AP*CP*(PGP))-3') Chain: B
Molecule details ›
Chain: B
Length: 9 nucleotides
Theoretical weight: 2.89 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 37.057Å b: 72.405Å c: 100.225Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.175 0.218
Expression systems:
  • Escherichia coli
  • Not provided