PDBe 3qmc

X-ray diffraction
2.1Å resolution

Structural Basis of Selective Binding of Nonmethylated CpG Islands by the CXXC Domain of CFP1

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
CXXC-type zinc finger protein 1 Chain: A
Molecule details ›
Chain: A
Length: 79 amino acids
Theoretical weight: 9.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9P0U4 (Residues: 161-222; Coverage: 10%)
Gene names: CFP1, CGBP, CXXC1, PCCX1, PHF18
5'-D(*GP*CP*CP*AP*CP*CP*GP*CP*TP*GP*GP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 12 nucleotides
Theoretical weight: 3.62 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-D(*GP*CP*CP*AP*GP*CP*GP*GP*TP*GP*GP*C)-3' Chain: C
Molecule details ›
Chain: C
Length: 12 nucleotides
Theoretical weight: 3.7 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 30.545Å b: 75.016Å c: 126.282Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.214 0.249
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided