› 3qkt

X-ray diffraction
1.9Å resolution

Rad50 ABC-ATPase with adjacent coiled-coil region in complex with AMP-PNP

Released:
DOI: 10.2210/pdb3qkt/pdb
PDB entry 3qkt coloured by chain, front view.
PDB entry 3qkt coloured by chain, side view.
PDB entry 3qkt coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
ABC ATPase signature helices in Rad50 link nucleotide state to Mre11 interface for DNA repair.
Williams GJ, Williams RS, Williams JS, Moncalian G, Arvai AS, Limbo O, Guenther G, SilDas S, Hammel M, Russell P, Tainer JA
Nat Struct Mol Biol 18 423-31 (2011)
PMID: 21441914

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157668 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA double-strand break repair Rad50 ATPase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 339 amino acids
Theoretical weight: 38.61 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: P58301 (Residues: 1-177, 185-882; Coverage: 38%)
Gene names: PF1167, rad50
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand ANP 4 x ANP
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 12.3.1
Spacegroup: P212121
Unit cell:
a: 83.39Å b: 108.517Å c: 150.366Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.192 0.244
Expression system: Escherichia coli

Quick links

Citations

46 review citations

Double-strand break end resection and repair pathway choice.
Symington et al. (2011)
45 more

5 mentions without citation

Archaeal genome guardians give insights into eukaryotic DNA replication and damage response proteins.
Shin et al. (2014)
4 more