PDBe 3qfc

X-ray diffraction
1.8Å resolution

Crystal Structure of Human NADPH-Cytochrome P450 (V492E mutant)

Released:
Source organism: Homo sapiens
Primary publication:
Structural basis for human NADPH-cytochrome P450 oxidoreductase deficiency.
Proc. Natl. Acad. Sci. U.S.A. 108 13486-91 (2011)
PMID: 21808038

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADPH--cytochrome P450 reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 618 amino acids
Theoretical weight: 70.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P16435 (Residues: 64-677; Coverage: 91%)
Gene names: CYPOR, POR
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN

Cofactor: Ligand FAD 2 x FAD

Cofactor: Ligand NAP 2 x NAP
1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 70.716Å b: 118.508Å c: 155.36Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.232 0.265
Expression system: Escherichia coli