› 3q4g

X-ray diffraction
2.4Å resolution

Structure of NAD synthetase from Vibrio cholerae

Released:
DOI: 10.2210/pdb3q4g/pdb
PDB entry 3q4g coloured by chain, front view.
PDB entry 3q4g coloured by chain, side view.
PDB entry 3q4g coloured by chain, top view.
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Entry authors: Anderson SM, Wawrzak Z, Onopriyenko O, Peterson SN, Anderson WF, Savchenko A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
ATP + deamido-NAD(+) + NH(3) = AMP + diphosphate + NAD(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191970 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NH(3)-dependent NAD(+) synthetase Chains: A, B
Molecule details ›
Chains: A, B
Length: 279 amino acids
Theoretical weight: 30.92 KDa
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KMW1 (Residues: 1-276; Coverage: 100%)
Gene names: VC_A0207, nadE
Sequence domains: NAD synthase
Structure domains: HUPs

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P3221
Unit cell:
a: 93.426Å b: 93.426Å c: 165.297Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.173 0.171 0.215
Expression system: Escherichia coli

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