› 3q27

X-ray diffraction
1.3Å resolution

Cyrstal structure of human alpha-synuclein (32-57) fused to maltose binding protein (MBP)

Released:
DOI: 10.2210/pdb3q27/pdb
PDB entry 3q27 coloured by chain, front view.
PDB entry 3q27 coloured by chain, side view.
PDB entry 3q27 coloured by chain, top view.
Source organisms:
Primary publication:
Structures of segments of α-synuclein fused to maltose-binding protein suggest intermediate states during amyloid formation.
Zhao M, Cascio D, Sawaya MR, Eisenberg D
Protein Sci 20 996-1004 (2011)
PMID: 21462277

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142535 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Alpha-synuclein; Maltose/maltodextrin-binding periplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 397 amino acids
Theoretical weight: 43.43 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 27-392; Coverage: 99%)
  • Canonical: P37840 (Residues: 32-57; Coverage: 19%)
Gene names: JW3994, NACP, PARK1, SNCA, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
2 bound ligands:
Ligand SO4 3 x SO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 57.511Å b: 49.951Å c: 59.342Å
α: 90° β: 92.19° γ: 90°
R-values:
R R work R free
0.132 0.131 0.156
Expression system: Escherichia coli

Quick links

Citations

16 citation in other articles

A soluble α-synuclein construct forms a dynamic tetramer.
Wang et al. (2011)
15 more

125 mentions without citation

Xenobiotic, bile acid, and cholesterol transporters: function and regulation.
Klaassen et al. (2010)
124 more