› 3pxl

X-ray diffraction
1.2Å resolution

Type-2 Cu-depleted fungus laccase from Trametes hirsuta

Released:
DOI: 10.2210/pdb3pxl/pdb
PDB entry 3pxl coloured by chain, front view.
PDB entry 3pxl coloured by chain, side view.
PDB entry 3pxl coloured by chain, top view.
Source organism: Trametes hirsuta
Entry authors: Polyakov KM, Fedorova TV, Kurzeev SA, Popov AN, Lamzin VS, Koroleva OV

Function and Biology Details

Reaction catalysed: 
4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109683 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Laccase Chain: A
Molecule details ›
Chain: A
Length: 499 amino acids
Theoretical weight: 53.63 KDa
Source organism: Trametes hirsuta
UniProt:
  • Canonical: B2L9C1 (Residues: 22-520; Coverage: 100%)
Gene name: Lac
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand CU 4 x CU
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P212121
Unit cell:
a: 52.653Å b: 77.051Å c: 130.216Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.153 0.179

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