PDBe 3pvu

X-ray diffraction
2.48Å resolution

Bovine GRK2 in complex with Gbetagamma subunits and a selective kinase inhibitor (CMPD101)

Released:
Source organism: Bos taurus

Function and Biology Details

Reaction catalysed:
ATP + [beta-adrenergic receptor] = ADP + [beta-adrenergic receptor] phosphate. 
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Beta-adrenergic receptor kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 695 amino acids
Theoretical weight: 80.58 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P21146 (Residues: 1-689; Coverage: 100%)
Gene names: ADRBK1, GRK2
Sequence domains:
Structure domains:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 340 amino acids
Theoretical weight: 37.42 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62871 (Residues: 1-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 74 amino acids
Theoretical weight: 8.41 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63212 (Residues: 1-68; Coverage: 96%)
Gene name: GNG2
Structure domains: Transducin (heterotrimeric G protein), gamma chain

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: C2
Unit cell:
a: 186.504Å b: 73.502Å c: 121.993Å
α: 90° β: 115.1° γ: 90°
R-values:
R R work R free
0.229 0.226 0.274
Expression system: Spodoptera frugiperda