PDBe 3pva

X-ray diffraction
2.8Å resolution

PENICILLIN V ACYLASE FROM B. SPHAERICUS

Released:

Function and Biology Details

Reaction catalysed:
Penicillin + H(2)O = a carboxylate + 6-aminopenicillanate. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Penicillin acylase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 335 amino acids
Theoretical weight: 37.19 KDa
Source organism: Lysinibacillus sphaericus
UniProt:
  • Canonical: P12256 (Residues: 4-338; Coverage: 99%)
Sequence domains: Linear amide C-N hydrolases, choloylglycine hydrolase family
Structure domains: Penicillin V Acylase; Chain A

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P1
Unit cell:
a: 47.4Å b: 129.6Å c: 156.7Å
α: 88.3° β: 83.4° γ: 84.6°
R-values:
R R work R free
0.211 0.211 0.243