› 3pu9

X-ray diffraction
1.55Å resolution

Crystal structure of serine/threonine phosphatase Sphaerobacter thermophilus DSM 20745

Released:
DOI: 10.2210/pdb3pu9/pdb
PDB entry 3pu9 coloured by chain, front view.
PDB entry 3pu9 coloured by chain, side view.
PDB entry 3pu9 coloured by chain, top view.
Source organism: Sphaerobacter thermophilus DSM 20745
Entry authors: Nocek B, Tesar C, Clancy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112274 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PPM-type phosphatase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 242 amino acids
Theoretical weight: 25.61 KDa
Source organism: Sphaerobacter thermophilus DSM 20745
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D1C2D8 (Residues: 1-239; Coverage: 100%)
Gene name: Sthe_0969
Sequence domains: Stage II sporulation protein E (SpoIIE)
Structure domains: PPM-type phosphatase domain

Ligands and Environments

2 bound ligands:
Ligand MG 4 x MG
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 60.168Å b: 54.713Å c: 63.262Å
α: 90° β: 99.51° γ: 90°
R-values:
R R work R free
0.183 0.178 0.217
Expression system: Escherichia coli BL21(DE3)

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