› 3pj7

X-ray diffraction
1.85Å resolution

Crystal structure of far-red fluorescent protein Katushka crystallized at pH 8.5

Released:
DOI: 10.2210/pdb3pj7/pdb
PDB entry 3pj7 coloured by chain, front view.
PDB entry 3pj7 coloured by chain, side view.
PDB entry 3pj7 coloured by chain, top view.
Source organism: Entacmaea quadricolor
Primary publication:
Crystallographic study of red fluorescent protein eqFP578 and its far-red variant Katushka reveals opposite pH-induced isomerization of chromophore.
Pletneva NV, Pletnev VZ, Shemiakina II, Chudakov DM, Artemyev I, Wlodawer A, Dauter Z, Pletnev S
Protein Sci 20 1265-74 (2011)
PMID: 21563226

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124780 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fluorescent protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 229 amino acids
Theoretical weight: 25.99 KDa
Source organism: Entacmaea quadricolor
Expression system: Escherichia coli
UniProt:
  • Canonical: H3JQU6 (Residues: 1-231; Coverage: 100%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand NRQ 4 x NRQ

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: I41
Unit cell:
a: 161.353Å b: 161.353Å c: 74.486Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.196 0.252
Expression system: Escherichia coli

Quick links

Citations

14 citation in other articles

Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics.
Akerboom et al. (2013)
13 more

2 mentions without citation

Structure-guided wavelength tuning in far-red fluorescent proteins.
Ng et al. (2016)
1 more