› 3pit

X-ray diffraction
1.55Å resolution

Crystal structure of M-RasD41E in complex with GppNHp (type 2)

Released:
DOI: 10.2210/pdb3pit/pdb
PDB entry 3pit coloured by chain, front view.
PDB entry 3pit coloured by chain, side view.
PDB entry 3pit coloured by chain, top view.
Source organism: Mus musculus
Entry authors: Muraoka S, Matsumoto K, Shima F, Hu L, Ijiri Y, Hirai R, Liao J, Kataoka T

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126703 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related protein M-Ras Chain: A
Molecule details ›
Chain: A
Length: 183 amino acids
Theoretical weight: 20.85 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: O08989 (Residues: 1-178; Coverage: 86%)
Gene names: Mras, Xras
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand GNP 1 x GNP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P212121
Unit cell:
a: 47.111Å b: 89.646Å c: 41.273Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.185 0.213
Expression system: Escherichia coli

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