› 3pf9

X-ray diffraction
1.75Å resolution

Crystal structure of the Lactobacillus johnsonii cinnamoyl esterase LJ0536 S106A mutant

Released:
DOI: 10.2210/pdb3pf9/pdb
PDB entry 3pf9 coloured by chain, front view.
PDB entry 3pf9 coloured by chain, side view.
PDB entry 3pf9 coloured by chain, top view.
Source organism: Lactobacillus johnsonii
Primary publication:
An inserted α/β subdomain shapes the catalytic pocket of Lactobacillus johnsonii cinnamoyl esterase.
Lai KK, Stogios PJ, Vu C, Xu X, Cui H, Molloy S, Savchenko A, Yakunin A, Gonzalez CF
PLoS One 6 e23269 (2011)
PMID: 21876742

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112587 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine aminopeptidase S33 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 270 amino acids
Theoretical weight: 30.01 KDa
Source organism: Lactobacillus johnsonii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D3YEX6 (Residues: 1-249; Coverage: 100%)
Gene name: FEE39_08260
Sequence domains: Serine aminopeptidase, S33
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand NA 4 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: C2221
Unit cell:
a: 72.74Å b: 85.702Å c: 81.889Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.145 0.142 0.199
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

21 citation in other articles

The metagenome-derived enzymes LipS and LipT increase the diversity of known lipases.
Chow et al. (2012)
20 more

3 mentions without citation

An inserted α/β subdomain shapes the catalytic pocket of Lactobacillus johnsonii cinnamoyl esterase.
Lai et al. (2011)
2 more