› 3pbf

X-ray diffraction
1.8Å resolution

Surfactant Protein-A neck and carbohydrate recognition domain (NCRD) complexed with glycerol

Released:
DOI: 10.2210/pdb3pbf/pdb
PDB entry 3pbf coloured by chain, front view.
PDB entry 3pbf coloured by chain, side view.
PDB entry 3pbf coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Crystallographic complexes of surfactant protein A and carbohydrates reveal ligand-induced conformational change.
Shang F, Rynkiewicz MJ, McCormack FX, Wu H, Cafarella TM, Head JF, Seaton BA
J Biol Chem 286 757-65 (2011)
PMID: 21047777

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140125 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pulmonary surfactant-associated protein A Chain: A
Molecule details ›
Chain: A
Length: 148 amino acids
Theoretical weight: 16.67 KDa
Source organism: Rattus norvegicus
Expression system: Trichoplusia
UniProt:
  • Canonical: P08427 (Residues: 101-248; Coverage: 65%)
Gene names: Sftp-1, Sftp1, Sftpa, Sftpa1
Sequence domains: Lectin C-type domain
Structure domains: Mannose-Binding Protein A, subunit A

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P63
Unit cell:
a: 97.376Å b: 97.376Å c: 44.905Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.207 0.223
Expression system: Trichoplusia

Quick links

Citations

5 review citations

Surfactant and its role in the pathobiology of pulmonary infection.
Glasser et al. (2012)
4 more

1 mention without citation

Crystallographic complexes of surfactant protein A and carbohydrates reveal ligand-induced conformational change.
Shang et al. (2011)