PDBe 3p9k

X-ray diffraction
2.25Å resolution

Crystal structure of perennial ryegrass LpOMT1 complexed with S-adenosyl-L-homocysteine and coniferaldehyde

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Caffeic acid O-methyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 364 amino acids
Theoretical weight: 39.15 KDa
Source organism: Lolium perenne
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9ZTU2 (Residues: 1-360; Coverage: 100%)
Gene name: OMT1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21
Unit cell:
a: 95.375Å b: 85.062Å c: 98.586Å
α: 90° β: 111.75° γ: 90°
R-values:
R R work R free
0.232 0.232 0.262
Expression system: Escherichia coli BL21(DE3)