› 3p2n

X-ray diffraction
1.95Å resolution

Discovery and structural characterization of a new glycoside hydrolase family abundant in coastal waters that was annotated as 'hypothetical protein'

Released:
DOI: 10.2210/pdb3p2n/pdb
PDB entry 3p2n coloured by chain, front view.
PDB entry 3p2n coloured by chain, side view.
PDB entry 3p2n coloured by chain, top view.
Source organism: Zobellia galactanivorans
Primary publication:
Discovery and structural characterization of a novel glycosidase family of marine origin.
Rebuffet E, Groisillier A, Thompson A, Jeudy A, Barbeyron T, Czjzek M, Michel G
Environ Microbiol 13 1253-70 (2011)
PMID: 21332624

Function and Biology Details

Reaction catalysed: 
Hydrolysis of the 1,3-alpha-L-galactosidic linkages of neoagaro-oligosaccharides that are smaller than a hexamer, yielding 3,6-anhydro-L-galactose and D-galactose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123277 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3,6-anhydro-alpha-L-galactosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 408 amino acids
Theoretical weight: 46.16 KDa
Source organism: Zobellia galactanivorans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F0V1E3 (Residues: 1-408; Coverage: 100%)
Gene names: Zg4663, ahgA, zobellia_4663
Sequence domains: Glycosyl hydrolases family 43
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1, ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 72.99Å b: 96.803Å c: 126.836Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.17 0.213
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Agar degradation by microorganisms and agar-degrading enzymes.
Chi et al. (2012)
8 more

2 mentions without citation

Environmental and gut bacteroidetes: the food connection.
Thomas et al. (2011)
1 more