PDBe 3ow5

X-ray diffraction
1.8Å resolution

Crystal structure of the Y200A mutant of gamma carbonic anhydrase from Methanosarcina thermophila

Source organism: Methanosarcina thermophila
Entry authors: Domsic JF, Robbins AH, McKenna R

Function and Biology Details

Reaction catalysed:
H(2)CO(3) = CO(2) + H(2)O. 
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Carbonic anhydrase Chain: A
Molecule details ›
Chain: A
Length: 213 amino acids
Theoretical weight: 22.8 KDa
Source organism: Methanosarcina thermophila
Expression system: Escherichia coli
  • Canonical: P40881 (Residues: 35-247; Coverage: 100%)
Sequence domains:
Structure domains: Hexapeptide repeat proteins

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P213
Unit cell:
a: 83.498Å b: 83.498Å c: 83.498Å
α: 90° β: 90° γ: 90°
R R work R free
0.13 0.128 0.152
Expression system: Escherichia coli