PDBe 3otm

X-ray diffraction
1.5Å resolution

Crystal structures of wild-type gamma-carbonic anhydrase from Methanosarcina thermophila

Released:
Source organism: Methanosarcina thermophila
Entry authors: Domsic JF, Robbins AH, McKenna R

Function and Biology Details

Reaction catalysed:
H(2)CO(3) = CO(2) + H(2)O. 
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbonic anhydrase Chain: A
Molecule details ›
Chain: A
Length: 212 amino acids
Theoretical weight: 22.76 KDa
Source organism: Methanosarcina thermophila
Expression system: Escherichia coli
UniProt:
  • Canonical: P40881 (Residues: 36-247; Coverage: 100%)
Sequence domains:
Structure domains: Hexapeptide repeat proteins

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P213
Unit cell:
a: 83.521Å b: 83.521Å c: 83.521Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.137 0.136 0.156
Expression system: Escherichia coli