› 3ot9

X-ray diffraction
1.75Å resolution

Phosphopentomutase from Bacillus cereus bound to glucose-1,6-bisphosphate

Released:
DOI: 10.2210/pdb3ot9/pdb
PDB entry 3ot9 coloured by chain, front view.
PDB entry 3ot9 coloured by chain, side view.
PDB entry 3ot9 coloured by chain, top view.
Source organism: Bacillus cereus ATCC 14579
Primary publication:
Bacillus cereus phosphopentomutase is an alkaline phosphatase family member that exhibits an altered entry point into the catalytic cycle.
Panosian TD, Nannemann DP, Watkins GR, Phelan VV, McDonald WH, Wadzinski BE, Bachmann BO, Iverson TM
J Biol Chem 286 8043-8054 (2011)
PMID: 21193409

Function and Biology Details

Reaction catalysed: 
Alpha-D-ribose 1-phosphate = D-ribose 5-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182450 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphopentomutase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 399 amino acids
Theoretical weight: 44.59 KDa
Source organism: Bacillus cereus ATCC 14579
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q818Z9 (Residues: 2-394; Coverage: 100%)
Gene names: BC_4087, deoB
Sequence domains: Metalloenzyme superfamily
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MN 9 x MN
Ligand GOL 3 x GOL
Ligand G16 1 x G16
1 modified residue:
Ligand TPO 3 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21
Unit cell:
a: 91.871Å b: 76.582Å c: 107.051Å
α: 90° β: 108.73° γ: 90°
R-values:
R R work R free
0.164 0.162 0.19
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

The Prodigal Compound: Return of Ribosyl 1,5-Bisphosphate as an Important Player in Metabolism.
Hove-Jensen et al. (2019)
16 other articles

4 mentions without citation

Divergence and convergence in enzyme evolution.
Galperin et al. (2012)
3 more