› 3osg

X-ray diffraction
2Å resolution

The structure of protozoan parasite Trichomonas vaginalis Myb2 in complex with MRE-1-12 DNA

Released:
DOI: 10.2210/pdb3osg/pdb
PDB entry 3osg coloured by chain, front view.
PDB entry 3osg coloured by chain, side view.
PDB entry 3osg coloured by chain, top view.
Source organism: Trichomonas vaginalis
Primary publication:
Molecular basis of the recognition of the ap65-1 gene transcription promoter elements by a Myb protein from the protozoan parasite Trichomonas vaginalis.
Jiang I, Tsai CK, Chen SC, Wang SH, Amiraslanov I, Chang CF, Wu WJ, Tai JH, Liaw YC, Huang TH
Nucleic Acids Res 39 8992-9008 (2011)
PMID: 21771861

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116671 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
MYB21 Chains: A, D
Molecule details ›
Chains: A, D
Length: 126 amino acids
Theoretical weight: 14.89 KDa
Source organism: Trichomonas vaginalis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58HP3 (Residues: 40-156; Coverage: 65%)
Sequence domains: Myb-like DNA-binding domain
Structure domains: Homeodomain-like
5'-D(*AP*AP*AP*TP*AP*TP*CP*GP*TP*TP*AP*T)-3' Chains: B, E
Molecule details ›
Chains: B, E
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: Trichomonas vaginalis
Expression system: Not provided
5'-D(*AP*TP*AP*AP*CP*GP*AP*TP*AP*TP*TP*T)-3' Chains: C, F
Molecule details ›
Chains: C, F
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: Trichomonas vaginalis
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P21
Unit cell:
a: 40.115Å b: 127.532Å c: 41.473Å
α: 90° β: 100.23° γ: 90°
R-values:
R R work R free
0.193 0.19 0.233
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Trichomonas vaginalis: pathogenicity and potential role in human reproductive failure.
Mielczarek et al. (2016)
2 more

2 mentions without citation

Identification and characterization of wheat drought-responsive MYB transcription factors involved in the regulation of cuticle biosynthesis.
Bi et al. (2016)
1 more